DCBLD2, Recombinant, Human, aa67-528 (CLCP1, ESDN, Iscoidin, CUB, LCCL Domain Containing Protein 2, Endothelial and Smooth Muscle Cell-derived Neuropilin-li)
Product Sizes
50ug
£900.00
145786-50UG
About this Product
- SKU:
- 145786
- Extra Details:
- DCBLD2 (discoidin, CUB, and LCCL domain containing protein 2), also known as CLCP1 and ESDN, is a 100130kD glycoprotein that bears structural resemblance to the Neuropilin family of molecules. Mature human DCBLD2 is a type I transmembrane protein that consists of a 462 amino acid (aa) extracellular region (ECD), a 21 aa transmembrane segment, and a 226 aa cytoplasmic domain (1, 2). Within the ECD, human DCBLD2 shares ~88% aa sequence identity with mouse and rat DCBLD2. Alternative splicing generates an isoform that contains a 14 aa insertion in the cytoplasmic domain. DCBLD2 is expressed on vascular smooth muscle cells (VSMC), particularly in regions of arteriosclerosis and vascular injury (2, 3). It is upregulated on PDGFBB stimulated VSMC and inhibits PDGFBB induced VSMC proliferation and migration (24). It is also expressed on platelets and inhibits clot formation in a zebrafish model of thrombosis (5). Compared to normal tissue, DCBLD2 expression is downregulated in some gastric and neuroendocrine tumors but is upregulated in some metastatic lung cancers (1, 6, 7). It can be tyrosine phosphorylated in response to EGF R signaling and can inhibit colony formation and tumor cell invasiveness (6, 8, 9). Its direct association with Semaphorin 4B induces DCBLD2 degradation (10). Source: Recombinant protein corresponding to aa67-528 from human DCBLD2/ESDN, fused to 6-his tag at C-terminal, NS0-derived. Molecular Weight: ~80-90kD Endotoxin: <0.01EU/1ug (LAL method) Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute in sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Molecular Weight:
- 8090
- Physical State:
- Supplied as a lyophilized powder in PBS. BSA free. Reconstitute in 300ug/ml PBS.
- Purity:
- ~95% (SDS-PAGE)
- Shipping Conditions:
- Blue Ice
- Storage Conditions:
- -20[o]C
- Supplier:
- United States Biological
- Type:
- Proteins, Peptides, Small Molecules & Other Biomolecules
