CD137, Fc Chimera, Recombinant, Rat, aa24-188 (4-1BB, TNFRSF9, ILA)
Product Sizes
50ug
£900.00
145778-50UG
About this Product
- SKU:
- 145778
- Extra Details:
- 4-1BB, also known as CD137 and TNFRSF9, is an ~30kD transmembrane glycoprotein in the TNF receptor superfamily. 4-1BB functions in the development and activation of multiple immune cells (1). Mature rat consists of a 166 amino acid (aa) extracellular domain (ECD) with four TNFR cysteine-rich repeats, a 21aa transmembrane segment, and a 48aa cytoplasmic domain. Within the ECD, rat 4-1BB shares 60% and 79% aa sequence identity with human and mouse 4-1BB, respectively. 4-1BB is expressed as a disulfidelinked homodimer on various populations of activated T cells including CD4+, CD8+, memory CD8+, NKT, and regulatory T cells (2-5) as well as on myeloid and mast cell progenitors, dendritic cells, mast cells, and bacterially infected osteoblasts (6-9). It binds with high affinity to the transmembrane 4-1BB Ligand/TNFSF9 which is expressed on antigen presenting cells and myeloid progenitor cells (6, 10). This interaction co-stimulates the proliferation, activation, and/or survival of the 4-1BB expressing cell (2-5, 10). It can also enhance the activationinduced cell death of repetitively stimulated T cells (10). Mice lacking 41BB show augmented T cell activation, perhaps due to its absence on regulatory T cells (11). 41BB can associate with OX40 on activated T cells, forming a complex that responds to either ligand and inhibits Treg and CD8+ T cell proliferation (12). Reverse signaling through 41BB Ligand inhibits the development of dendritic cells, B cells, and osteoclasts (6, 9) but supports mature dendritic cell survival and co-stimulates the proliferation and activation of mast cells (7, 8). 4-1BB activation enhances CD8+ T cell and NK cell mediated anti-tumor immunity (13). It also contributes to the development of inflammation in high fat dietinduced metabolic syndrome (14). Soluble forms of 4-1BB and 4-1BB Ligand circulate at elevated levels in the serum of rheumatoid arthritis and hematologic cancer patients, respectively (15, 16). Source: Recombinant protein corresponding to aa24-188 from rat 4-1BB/TNFRSF9 Fc Chimera, NS0-derived. Molecular Weight: ~60-65kD Endotoxin: <0.01EU/1ug (LAL method) Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Molecular Weight:
- 6065
- Physical State:
- Supplied as a lyophilized powder in PBS. BSA free. Reconstitute in 100ug/ml PBS.
- Purity:
- ~95% (SDS-PAGE)
- Shipping Conditions:
- Blue Ice
- Storage Conditions:
- -20[o]C
- Type:
- Proteins, Peptides, Small Molecules & Other Biomolecules:Recombinant Proteins
