TLR11 (Toll-like Receptor 11, Gm287)
Product Sizes
100ug
£748.00
145631-100UG
About this Product
- SKU:
- 145631
- Application:
- Flow Cytometry
- translate.label.attr.clone:
- 786404
- Clonality:
- Monoclonal
- Extra Details:
- TLR11 is a type I transmembrane receptor of the Toll-like receptor family that is primarily expressed in epithelial cells, but also in dendritic cells and macrophages. The 926 amino acid (aa) mouse TLR11 transcript encodes a 30aa signal sequence, a 691aa extracellular domain with 10 leucine-rich repeats and 9 potential N-glycosylation sites, a 21aa transmembrane domain, and a 184aa cytoplasmic domain with a TIR domain. Within the region used as an immunogen, mouse and rat TLR11 share 86% aa sequence identity. Human TLR11 is a pseudogene that is not expressed. TLR11 resides in the endoplasmic reticulum (ER), interacts with the multispan ER protein UNC93B1, recognizes profilin-like proteins on Toxoplasma gondii and other intracellular parasites, and activates dendritic cell IL-12 production. Applications: Suitable for use in Flow Cytometry and ELISA. Other applications not tested. Recommended Dilution: Flow Cytometry: 2.5ug/10e6 ells Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to aa209-325 mouse TLR11 expressed in E. coli (UniProt # Q6R5P0).
- Isotype:
- IgG2b
- Physical State:
- Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
- Purity:
- Purified by Protein A or G affinity chromatography.
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes mouse TLR11 in direct ELISAs.
- Source:
- rat
- Storage Conditions:
- -20[o]C
- Supplier:
- United States Biological
- Type:
- Antibodies:Monoclonal Antibody
