SRPK3 (Ser/Arg-rich Protein Specific kinase 3, MSSK1, Muscle-specific Serine Kinase 1, STK23, Ser/Thr Kinase 23)
Product Sizes
100ug
£761.00
145614-100UG
About this Product
- SKU:
- 145614
- Application:
- Western Blot, Immunohistochemistry
- translate.label.attr.clone:
- 722516
- Clonality:
- Monoclonal
- Extra Details:
- SRPK3 (Ser/Arg-rich protein specific kinase 3), also called MSSK-1 (muscle-specific serine kinase 1) or STK23 (Ser/Thr kinase 23) is a 59kD (predicted) cytoplasmic member of the CMGC Ser/Thr protein kinase family. SRPK3 is principally expressed in heart and skeletal muscle. SRPKs selectively phosphorylate Ser on RS domain-containing proteins, initiating mRNA splicing and maturation. The 533 amino acid (aa) human SRPK3 contains a split kinase domain (aa79-292 and 347-531) separated by a hinge region. A 491aa isoform shows an alternate translation start site at aa43. Within the region used as an immunogen, human SRPK3 shares 87% and 86% aa identity with mouse and rat SRPK3, respectively. Applications: Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1ug/ml Immunohistochemistry: 8-25ug/ml IOptimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to Arg247-Ser316 from human SRPK3, expressed in E. coli .
- Isotype:
- IgG1
- Physical State:
- Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
- Purity:
- Purified by Protein G affinity chromatography.
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes human SRPK3.
- Source:
- human
- Storage Conditions:
- -20[o]C
- Supplier:
- United States Biological
- Type:
- Antibodies:Monoclonal Antibody
