SPOCK2 (Testican 2, KIAA0275) (Biotin)
Product Sizes
50ug
£914.00
145613-50UG
About this Product
- SKU:
- 145613
- Application:
- Western Blot
- Clonality:
- Polyclonal
- Extra Details:
- Testican 2 (also SPOCK2) is a 60-140kD, secreted multidomain proteoglycan that belongs to the BM-40/SPARC family of extracellular proteins. It is expressed by neurons (both excitatory and GABAergic) plus brain endothelial cells, and appears to abrogate testican 1 and 3 mediated inhibition of MT1- and MT3-MMP activity. Mature human Testican 2 is 402 amino acids (aa) in length (aa23-424). It contains a unique 66aa N terminus, followed by a Kazal-like follistatin domain (aa89-192), a collagen-binding domain (aa210-219), a Ca++ binding region, one thyroglobulin-like segment (aa310-376) and an acidic C-terminus that contains two potential GAG attachment sites at Ser383 and Ser388. The wide variation in native MW reflects the capabilities of differing cells to add glycan adducts to the molecule. Typically, it appears to lie belween 80-125kD. There are potential splice variants. One shows an alternative start site at Met110, a second shows a deletion of aa64-66, while a third contains a 14aa substitution for aa64-242. Mature human Testican 2 is 94% aa identical to mouse and rat Testican 2. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 0.1ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Host:
- Goat
- Immunogen:
- Recombinant protein corresponding to Glu23-Trp424 from human SPOCK2, NS0-derived.
- Isotype:
- IgG
- Physical State:
- Supplied as a lyophilized powder in PBS, BSA. Labeled with Biotin. Reconstitute in Tris-buffer saline, pH 7.3.
- Purity:
- Purified by immunoaffinity chromatography.
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes human Testican 2/SPOCK2 <5% with recombinant human Testican 1 and rhTestican 3.
- Source:
- human
- Storage Conditions:
- -20[o]C
- Supplier:
- United States Biological
- Type:
- Antibodies:Polyclonal Antibody
