NQO1 (NQO-1, NADPH Quinone Acceptor Oxidoreductase 1, Azoreductase, DHQU, DIA4, Diaphorase-4, Dioxin-inducible 1, DTD, Menadione Reductase, NMOR1, QR1, Quinone Reductase 1)
Product Sizes
100ug
£748.00
145556-100UG
About this Product
- SKU:
- 145556
- Application:
- Western Blot
- translate.label.attr.clone:
- 844142
- Clonality:
- Monoclonal
- Extra Details:
- NQO1 (NADPH Quinone acceptor Oxidoreductase 1; also DTD and Menadione reductase) is a 3033kD cytoplasmic member of the NAD(P)H dehydrogenase family of enzymes. It is widely expressed, being found in adipocytes, endothelial cells and hepatocytes. NQO1 serves as a cytoprotective molecule, reducing quinones (atmospheric benzene and esrtrogen compounds) and nitroaromatics without consuming sulfhydryl compounds. It also acts as a backup superoxide scavenger to SOD. NQO1 reportedly binds and stabilizes key cell protection molecules such as p53, thus acting as a gatekeeper for proteosomemediated protein turnover. Human NQO1 is 274 amino acids (aa) in length. It contains an FMN reductase domain (aa5212) and two utilized Lys acetylation sites. NQO1 functions as a noncovalent homodimer. There are three potential at Met22, while two others show a deletion of aa141174 and 102139, respectively. Full length human NQO1 shares 86% aa sequence identity with mouse NQO1. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 0.5ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to aa1-274 from human NQO-1, expressed in E. coli.
- Isotype:
- IgG2b
- Physical State:
- Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
- Purity:
- Purified by Protein A affinity chromatography.
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes human NQO1.
- Source:
- human
- Storage Conditions:
- -20[o]C
- Type:
- Antibodies:Monoclonal Antibody
