Glycoprotein V (GP-V, CD42d, GP5, GPV)
Product Sizes
100ug
£876.00
145481-100UG
About this Product
- SKU:
- 145481
- Application:
- Western Blot
- Clonality:
- Polyclonal
- Extra Details:
- GP-V (platelet glycoprotein V; also CD42d) is a 78-88kD member of the leucine-rich (LR) repeat family of proteins. It is expressed on the surface of platelets where it noncovalently interacts with three additional proteins to form a heteromeric complex that serves as a receptor for both thrombin and von Willebrand factor. In addition, GP-V is normally a receptor for collagen. Following receptor complex activation due to thrombin cleavage of GP-V, the collagen-binding activity of GP-V is lost, limiting thrombus growth at sites of clot formation. Mature mouse GP-V is a 551 amino acid (aa) type I transmembrane glycoprotein. In its 506 aa extracellular domain (aa17-522), GP-V contains 16 LR repeats (aa17-474), and terminates with a short 24aa cytoplasmic tail. Based on human, mouse GP-V might be naturally cleaved by thrombin and ADAM17, generating soluble fragments that range from 70-82kD in size. Over aa17-522, mouse GP-V shares 83% and 70% aa sequence identity with rat and human GP-V, respectively. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1ug/ml Immunocytochemistry: 5-15ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Host:
- Sheep
- Immunogen:
- Recombinant protein corresponding to aa17-522 from mouse Glycoprotein V, NS0-derived.
- Isotype:
- IgG
- Physical State:
- Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
- Purity:
- Purified by immunoaffinity chromatography.
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes mouse Glycoprotein V/CD42d in direct ELISAs and Western blots.
- Source:
- mouse
- Storage Conditions:
- -20[o]C
- Supplier:
- United States Biological
- Type:
- Antibodies:Polyclonal Antibody
