CRTAC1 (Cartilage Acidic Protein 1, ASPIC1, CEP-68, CEP68)
Product Sizes
100ug
£748.00
145440-100UG
About this Product
- SKU:
- 145440
- Application:
- Western Blot
- translate.label.attr.clone:
- 755339
- Clonality:
- Monoclonal
- Extra Details:
- CRTAC1 (cartilage acidic protein 1; also CEP-68) is a 95-105kD member of a novel family of EGF domain-containing proteins. It is secreted by articular chondrocytes and may play a role in either cartilage matrix organization, or cell-matrix adhesion. Mature human CRTAC1 is 634 amino acids (aa) in length. It contains four FG-GAP (PheGly-GlyAlaPro) domains (aa46-437) and one EGF-like motif (aa559-605). Multiple splice forms exist. There are two alternate start sites at Met9 and Met211 that may be accompanied by a 39aa substitution for the C-terminal 55 aa, or an 84aa substitution for aa45-661. Over aa28-661, human CRTAC1 shares 91% aa identity with mouse CRTAC1. This form in mouse, however, is more equivalent to the human isoform that shows a C-terminal 39aa substitution. In this case, there is 95% aa identity between mouse and human. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 2ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to aa28-661 from human CRTAC1, NS0-derived.
- Isotype:
- IgG2b
- Physical State:
- Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
- Purity:
- Purified by Protein A affinity chromatography.
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes human CRTAC1.
- Source:
- human
- Storage Conditions:
- -20[o]C
- Supplier:
- United States Biological
- Type:
- Antibodies:Monoclonal Antibody
