Intelectin-2 (Endothelial Lectin 2, HL-2, Intelectin-b, Itln1b, ITLN2, Itlnb)
Product Sizes
100ug
£748.00
145419-100UG
About this Product
- SKU:
- 145419
- Application:
- Western Blot, Immunohistochemistry
- translate.label.attr.clone:
- 747421
- Clonality:
- Monoclonal
- Extra Details:
- Intelectin-2 (endothelial lectin 2; also ITLN-2 and HL-2) is a 40kD, likely Ca-dependent carbohydrate-binding lectin that is a member of the X-lectin family of molecules. It is secreted by intestinal Paneth cells and appears to participate in fungal microbe recognition. The Intelectin-2 precursor is 325 amino acids (aa) in length. It contains a 26aa signal sequence and a 299aa mature region. Although mouse Intelectin-2 is reportedly GPI-linked and mouse and human Intelectin-2 show considerable C-terminal aa identity, it is not known if human Intelectin-2 is also GPI-linked. There is one fibrinogen-related domain in the mature molecule (aa45-210). One potential splice variant is reported that shows a premature truncation after Gly264. Over aa20-298, mouse Intelectin-2 shows 78% aa identity to human Intelectin-2. Applications: Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 2ug/ml Immunohistochemistry: 8-25ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Host:
- Rat
- Immunogen:
- Recombinant protein corresponding to aa20-298 from mouse Intelectin-2, expressed in E. coli (UniProt # Q80ZA0).
- Isotype:
- IgG1
- Physical State:
- Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
- Purity:
- Purified by Protein A or G affinity chromatography.
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes mouse Intelectin2. Species Crossreactivity: 100% with recombinant mouse Intelectin1. In direct ELISAs, ~25% with recombinant human Intelectin2, and ~15% with rhIntelectin1.
- Source:
- mouse
- Storage Conditions:
- -20[o]C
- Supplier:
- United States Biological
- Type:
- Antibodies:Monoclonal Antibody
