Calponin 1 (Calponin H1, CNN1, Sm-Calp, SMCC, Calponin-1, Calcium Calmodulin-binding Troponin T-like protein, Calponin basic, CaP)
Product Sizes
100ug
£825.00
145404-100UG
About this Product
- SKU:
- 145404
- Application:
- Immunohistochemistry
- Clonality:
- Polyclonal
- Extra Details:
- CNN1 (calponin1 [calcium and calmodulinbinding troponin Tlike protein]; also calponin basic, CaP and calponin H1) is a 3236kD cytoplasmic member of the calponin family of proteins. Although reportedly expressed in fibroblasts and endothelial cells, it actually appears to be restricted to smooth muscle and smooth musclelike cells such as myoepithalium and myofibroblasts in the adult. CNN1 interacts with Factin in a phosphorylationdependent manner. When nonphosphorylated, CNN1 blocks actomyosin ATPase activity, contributing to the stabilization of actin stress fiber bundles. Thus, CNN1 expression inhibits cell motility and the formation of podosomes. Human CNN1 is 297 amino acids (aa) in length. It contains one CH/calponin homology domain (aa30127), and three consecutive calponinlike repeats (aa164268). The repeats are suggested to mediate actin binding. There are five potential Ser/Thr phosphorylation sites. Fulllength human CNN1 shares 97% aa sequence identity with mouse CNN1. Applications: Suitable for use in Immunohistochemistry and Immunocytochemistry. Other applications not tested. Recommended Dilution: Immunohistochemistry: 5-15ug/ml Immunocytochemistry: 5-15ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Host:
- Sheep
- Immunogen:
- Recombinant protein corresponding to Ser2-Ala297 from human Calponin 1, expressed in E. coli.
- Isotype:
- IgG
- Physical State:
- Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
- Purity:
- Purified by immunoaffinity chromatography.
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes human Calponin 1. <5% crossreactivity with recombinant human CNN3.
- Source:
- human
- Storage Conditions:
- -20[o]C
- Supplier:
- United States Biological
- Type:
- Antibodies:Polyclonal Antibody
