CCL19 (C-C Motif Chemokine 19, Small Inducible Cytokine A19, Macrophage Inflammatory Protein 3 beta, MIP-3-beta, Epstein-Barr Virus-induced Molecule 1 Ligand Chemokine, EBI1-ligand Chemokine, ELC, Beta Chemokine Exodus-3, CK beta-11, MIP3B, SCYA19, MGC34433)
Product Sizes
500ug
£466.00
143658-500UG
About this Product
- SKU:
- 143658
- Application:
- Western Blot, Immunohistochemistry
- translate.label.attr.clone:
- 14G2
- Clonality:
- Monoclonal
- Extra Details:
- The gene for CCL19 is one of several CC cytokine genes clustered on the p-arm of chromosome 9. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. CCL19 may play a role in normal lymphocyte recirculation and homing. It also plays an important role in trafficking of T cells in thymus, and in T cell and B cell migration to secondary lymphoid organs. It specifically binds to chemokine receptor CCR7. Applications: Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: ELISA (Sandwich): 2-4ug/ml (assuming 100ul/well), will detect recombinant human MIP-3beta when used with biotinylated antigen affinity purified anti-human MIP-3beta (M1202-67) as the detection antibody at a concentration of ~0.5-1ug/ml. Western Blot: 1-2ug/ml. When used in conjunction with compatible secondary reagents the detection limit for recombinant human MIP-3beta is 2-4ng/lane, under reducing conditions. Immunohistochemistry: This antibody stained PBMC. The primary antibody was incubated at 5ug/ml for one hour at RT followed by a fluorescent labeled secondary antibody. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to highly purified human CCL19 expressed in E. coli.
- Isotype:
- IgG1,k
- Physical State:
- Supplied as a lyophilized powder. Reconstitute with 500ul sterile ddH2O.
- Purity:
- Purified by Protein A affinity chromatography.
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes human CCL19.
- Source:
- human
- Storage Conditions:
- -20[o]C
- Supplier:
- United States Biological
- Type:
- Antibodies:Monoclonal Antibody
