Il6 (Interleukin-6, IL-6, B Cell Hybridoma Growth Factor, Interleukin HP-1, Il-6) (Biotin)
Product Sizes
50ug
£614.00
143631-50UG
About this Product
- SKU:
- 143631
- Application:
- Western Blot
- Clonality:
- Polyclonal
- Extra Details:
- Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 0.25-1ug/ml, in conjunction with I8428-30A as a capture antibody, allows the detection of at least 0.2-0.4ng/well of recombinant mouse IL-6. Western Blot: 0.1-0.2ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant mouse IL-6 is 1.5-3ng/lane, under either reducing or non-reducing conditions. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Host:
- Rabbit
- Immunogen:
- Recombinant protein corresponding to mouse IL-6, expressed in E. coli.
- Isotype:
- IgG
- Physical State:
- Supplied as a lyophilized powder from PBS. No preservative added. Labeled with Biotin. Reconstitute with sterile PBS, 0.1% BSA to 0.1-1mg/ml. Do not vortex.
- Purity:
- Purified by immunoaffinity chromatography. Endotoxin: ≤1EU/ug (LAL).
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes mouse IL-6.
- Source:
- mouse
- Storage Conditions:
- -20[o]C
- Supplier:
- United States Biological
- Type:
- Antibodies:Monoclonal Antibody
