IL10 (Interleukin-10, IL-10, Interleukin 10, Cytokine Synthesis Inhibitory Factor, CSIF)
Product Sizes
500ug
£466.00
143595-500UG
About this Product
- SKU:
- 143595
- Application:
- Western Blot
- translate.label.attr.clone:
- 14G8
- Clonality:
- Monoclonal
- Extra Details:
- The protein is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract. Applications: Suitable for use in ELISA, Western Blot and Neutralization. Other applications not tested. Recommended Dilution: Sandwich ELISA: 8-10ug/ml detects at least 1000pg/well of recombinant human IL-10 when used with biotinylated anti-human IL-10 (I8432-17) as the detection antibody at a concentration of ~0.5-1ug/ml. Indirect ELISA: 4-8ug/ml detects at least 196pg/ml of recombinant human IL-10. Western Blot: 0.25-0.50ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant human IL-10 is 2-4ng/lane, under reducing or non-reducing conditions. Neutralization: 38-57ug/ml yeilds one half of the maximal inhibition [ND50] of the biological activity oh human IL-10 (1mg/ml) Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Host:
- Mouse
- Immunogen:
- Recombinant human IL-10 expressed in E.coli.
- Isotype:
- IgG1,k
- Physical State:
- Supplied as a lyophilized powder from PBS. Reconstitute with 500ul of sterile ddH2O.
- Purity:
- Purified by Protein A affinity chromatography.
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes human IL-10.
- Source:
- human
- Storage Conditions:
- -20[o]C
- Type:
- Antibodies:Monoclonal Antibody
