sIL-2Ra, Recombinant, Human (Soluble IL-2 receptor, TAC-antigen, CD25 antigen)
Product Sizes
5ug
143502-5UG
About this Product
- SKU:
- 143502
- Extra Details:
- The IL-2 receptor system consists of three non-covalently linked subunits termed IL-2Rα, IL-2Rβ, and IL-2Rγ. The IL-2Rα is a type I transmembrane protein consisting of a 219 amino acid extracellular domain, a 19 amino acid transmembrane domain and a 13 amino acid intracellular domain, which is not involved in the transduction of IL-2 signals. Proteolytic processing of IL-2Rα releases the entire extracellular domain of IL-2Rα, thereby generating a 219 amino acid soluble protein called soluble IL-2Rα (sIL-2Rα). The homodimeric form binds IL-2 (KD=10mM) and facilitates IL-2 signaling. The secreted sIL-2Rα is expressed on leukemia cells, lymphoma cells, and newly activated T and B cells, as well as on approximately 10% of NK cells. Recombinant Human sIL-2 Receptor α is a 24.8 kDa protein containing 219 amino acid residues consisting of only the extracellular domain of IL-2Rα. As a result of glycosylation, Recombinant Human sIL-2 Receptor α migrates with an apparent molecular mass of approximately 40-50 kDa by SDS-PAGE gel, under reducing and non-reducing conditions. Recombinant protein corresponding to human sIL-2 Receptor, expressed in CHO cells. Biological Activity: Determined by its ability to increase the proliferation effect of IL-2 in murine CTLL-2 cells. In the presence of 1ng/ml of recombinant IL-2, the expected ED50 for this effect is between 0.5-1.5ug/ml. Amino Acid Sequence: ELCDDDPPEI PHATFKAMAY KEGTMLNCEC KRGFRRIKSG SLYMLCTGNS SHSSWDNQCQ CTSSATRNTT KQVTPQPEEQ KERKTTEMQS PMQPVDQASL PGHCREPPPW ENEATERIYH FVVGQMVYYQ CVQGYRALHR GPAESVCKMT HGKTRWTQPQ LICTGEMETS QFPGEEKPQA SPEGRPESET SCLVTTTDFQ IQTEMAATME TSIFTTEYQ Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile ddH2O. For longer term storage the protein solution should be stored with a carrier protein (eg. 0.1% BSA). Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Molecular Weight:
- 24.8
- Physical State:
- Supplied as a lyophilized powder. Reconstitute with sterile ddH2O to 0.1-1mg/ml
- Purity:
- ≥98% (SDS-PAGE gel and HPLC). Endotoxin: ≤0.1ng/ug (<1EU/ug)
- Shipping Conditions:
- Blue Ice
- Storage Conditions:
- -20[o]C
- Type:
- Proteins, Peptides, Small Molecules & Other Biomolecules:Recombinant Proteins
