Caveolin 1 (Caveolin-1, CAV, CAV1) (HRP)
Product Sizes
100ul
£748.00
137422-100UL
About this Product
- SKU:
- 137422
- Application:
- Western Blot
- translate.label.attr.clone:
- 13G25(D46G3)
- Clonality:
- Monoclonal
- Extra Details:
- The 21-24 kDa integral proteins, caveolins, are the principal structural components of the cholesterol/sphingolipid-enriched plasma membrane microdomain caveolae. Three members of the caveolin family (caveolin-1, -2, and -3) have been identified with different tissue distributions. Caveolins form hetero- and homo-oligomers that interact with cholesterol and other lipids. Caveolins are involved in diverse biological functions, including vesicular trafficking, cholesterol homeostasis, cell adhesion, and apoptosis, and are also implicated in neurodegenerative disease. Caveolins interact with multiple signaling molecules such as Ga subunit, tyrosine kinase receptors, PKCs, Src family tyrosine kinases, and eNOS. It is believed that caveolins serve as scaffolding proteins for the integration of signal transduction. Phosphorylation at Tyr14 is essential for caveolin association with SH2 or PTB domain-containing adaptor proteins such as GRB7. Phosphorylation at Ser80 regulates caveolin binding to the ER membrane and entry into the secretory pathway. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: 1:1000. Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Host:
- Rabbit
- Immunogen:
- Synthetic peptide corresponding to residues surrounding Glu20 of human caveolin-1.
- Isotype:
- IgG
- Physical State:
- Supplied as a liquid in 136mM sodium chloride, 2.6mM potassium chloride, 12mM sodium phosphate dibasic, pH 7.4, 2mg/ml BSA, 50% glycerol. Labeled with horseradish peroxidase (HRP).
- Purity:
- Purified
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes endogenous levels of total human Caveolin-1. Species Crossreactivity: rat, mouse, hamster, monkey, bovine and canine.
- Source:
- human
- Storage Conditions:
- -20[o]C
- Supplier:
- United States Biological
- Type:
- Antibodies:Monoclonal Antibody
