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Antibodies

CD3 (APC)

Product Sizes
100Tests
£672.00
059447-100TESTS
About this Product
SKU:
059447
Application:
Flow Cytometry
translate.label.attr.clone:
17A2
Clonality:
Monoclonal
Extra Details:
CD3 a member of the immunoglobulin superfamily of cell surface receptors, is comprised of five invariable chain ranging in size from 16-28kD and is closely associated with the T cell antigen receptor (TCR). It is expressed on all T cells of all mouse strains. CD3 plays a major role in signaling during antigen recognition, leading to T-cell activation. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 10ul labels 10e6 cells using mouse splenocytes. Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Host:
Rat
Immunogen:
T cell hybridoma D1
Isotype:
IgG2b
Physical State:
Supplied as a liquid in PBS, 0.5% BSA, 0.09% sodium azide. Labeled with Allophycocyanin (APC).
Purity:
Purified by Protein G affinity chromatography from hybridoma culture supernatant.
Shipping Conditions:
Blue Ice
Specificity:
Recognizes mouse TCR-associated CD3 complex that occurs on thymocytes and mature T cells. Has been shown to react with the epsilon chain of the CD3 complex (1).
Source:
mouse
Storage Conditions:
4[o]C Do Not Freeze
Supplier:
United States Biological
Type:
Antibodies:Monoclonal Antibody