NCAM-1 (Neural Cell Adhesion Molecule, CD56)
Product Sizes
500ug
£997.00
059442-500UG
About this Product
- SKU:
- 059442
- Application:
- Flow Cytometry, Western Blot
- translate.label.attr.clone:
- 26F2
- Clonality:
- Monoclonal
- Extra Details:
- Neural cell adhesion molecule 1 (NCAM-1) is a multifunctional member of the Ig superfamily. It belongs to a family of membrane-bound glycoproteins that are involved in Ca++ independent cell matrix and homophilic or heterophilic cell-cell interactions. NCAM-1 specifically binds to heparan sulfate proteoglycans (1), the extracellular matrix protein agrin (2), and several chondroitin sulfate proteoglycans that include neurocan and phosphocan (3). There are three main forms of human NCAM-1 that arise by alternate splicing. These are designated NCAM-120/NCAM-1 (761 amino acids [aa]), NCAM-140 (848 aa), and NCAM-180 (1120 aa). NCAM-120 is GPI-linked, while NCAM-140 and NCAM-180 are type I transmembrane glycoproteins (4‑6). Additional alternate splicing adds considerable diversity to all three forms, and extracellular proteolytic processing is possible for NCAM-180 (7‑8). NCAM-1 is synthesized as a 761 aa preproprecursor that contains a 19 aa signal sequence, a 722 aa GPI-linked mature region, and a 20 aa C-terminal prosegment (4). The molecule contains five C-2 type Ig-like domains and two fibronectin type-III domains. Human to mouse, NCAM-1 is 93% aa identical. NCAM-1 appears to be highly sialylated. The polysialyation of NCAM-1 reduces its adhesive property and increases its neurite outgrowth promoting features (9). NCAM-1 in the adult brain shows a decline of sialylation relative to earlier developmental periods. In regions that retain a high degree of neuronal plasticity, however, the adult brain continues to express polysialylation-NCAM-1, suggesting sialylation of NCAM-1 is involved in regenerative processes and synaptic plasticity (10‑13). Applications: Suitable for use in Flow Cytometry, Western Blot and ELISA. Other applications not tested. Recommended Dilutions: Flow Cytometry: 0.25ug/10e6 cells with human peripheral blood mononuclear cells and human NK cells. Western Blot: 1ug/ml using human brain (cerebellum) tissue and human brain (motor cortex) tissue under reducing conditions. ELISA (capture): 2-8ug/ml with 059443 as detection antibody. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to human NCAM‑1/CD56, expressed in mouse myeloma cell line NS0 cells.
- Isotype:
- IgG2b
- Physical State:
- Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 1ml sterile PBS.
- Purity:
- Purified by Protein A affinity chromatography from hybridoma culture supernatant.
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes human NCAM‑1/CD56. Does not react with recombinant human ALCAM, recombinant human BCAM, recombinant human EPCAM, recombinant human MCAM, recombinant human NCAM-1-L1, recombinant mouse MAdCAM-1, recombinant mouse NCAM-1-L1 or recombinant mouse OCAM. Species Crossreactivity: primate
- Source:
- human
- Storage Conditions:
- -20[o]C
- Supplier:
- United States Biological
- Type:
- Antibodies:Monoclonal Antibody
