Adenylate Cyclase III (ACIII, AC111, AC3)
Product Sizes
100ul
£607.00
059379-100UL
About this Product
- SKU:
- 059379
- Application:
- Western Blot, Immunofluorescence
- translate.label.attr.clone:
- 1A12
- Clonality:
- Monoclonal
- Extra Details:
- Trimeric G-proteins are a large and variable family of membrane receptors. On binding their specific ligand they activate specific members of the family of trimeric G-proteins which in turn activate other signalling enzymes. Adenylate cyclases are one of these downstream enzyme families which are activated by the GTP bound GαS subunits of trimeric G-proteins. Adenylate cyclases are responsible for the production of the important “second messenger” signaling molecule cyclic-AMP which in turn activates the cAMP dependent protein kinase. This kinase when activated phosphorylates numerous substrate molecules on serine or threonine residues and so alters their activity. There are several different adenylate cyclase genes and protein products with each have distinctly different distribution patterns in cells and tissues. The type III adenylate cyclase enzyme is specifically localized in the membranes surrounding neuronal cilia, and is activated by specific G-protein coupled receptors also located in cilia (1-5). Neuronal cilia express a variety of other receptors types and mediators of other signaling pathways and appear to function as a unique and complex neuronal sensory structure (1-5). For examples, the somatostatin 3 receptor, neuropeptide Y 2 receptor and melanin concentrating hormone receptor 1 are localized in neuronal cilia and the sonic hedgehog and Wnt signalling pathway act on neurons primarily through neuronal cilia (6). A cysteine residue was added to the N-terminus of 059379 to allow coupling to MBS-activated keyhole limpet hemocyanin. The antibody works on rat and mouse cells which express the same peptide and also on human cells, presumably because the corresponding peptide in the human AC3 sequence is the closely related peptide LATFPNGPSVTLPHQVVDNS. This antibody is an excellent marker of neuronal cilia in the brain and in cells in tissue culture. The antibody was recently utilized in a very high profile publication in the journal Cell (8). Applications: Suitable for use in Western Blot and Immunocytochemistry/Immunofluorescence. Not suitable for use in Immunohistochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 1:1000-1:2000 Immunocytochemistry (IF): 1:1000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Host:
- Mouse
- Immunogen:
- C-terminal peptide corresponding to aa1125-1144 from rat ACIII, PAAFPNGSSVTLPHQVVDNP, with a Cys added to the N-terminus to allow coupling to KLH.
- Isotype:
- IgG1
- Physical State:
- Supplied as a liquid in PBS, 5mM sodium azide, 50% glycerol.
- Purity:
- Purified by Protein G affinity chromatography.
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes rat ACIII. Species Crossreactivity: human and mouse
- Source:
- rat
- Storage Conditions:
- -20[o]C
- Supplier:
- United States Biological
- Type:
- Antibodies:Monoclonal Antibody
