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Proteins and Peptides

Eukaryotic Sclerostin (SOST), Recombinant, Human, His-Tag

Product Sizes
10ug
£416.00
057833-10UG
About this Product
SKU:
057833
Application:
Western Blot
Extra Details:
Recombinant protein corresponding to aa25-213 from human Eukaryotic Sclerostin, fused to His-Tag at N-terminal, expressed in 293F cells. Predicted Molecular Weight: 23kD Accurate Molecular Weight: 33-35kD Isoelectric Point: 9.9 Subcellular Location: Secreted, Extracellular matrix Amino Acid Sequence: GWQAFKNDAT EIIPELGEYP EPPPELENNK TMNRAENGGR PPHHPFETKD VSEYSCRELH FTRYVTDGPC RSAKPVTELV CSGQCGPARL LPNAIGRGKW WRPSGPDFRC IPDRYRAQRV QLLCPGGEAP RARKVRLVAS CKCKRLTRFH NQSELKDFGT EAARPQKGRK PRPRARSAKA NQAELENAY Applications: Suitable for use in ELISA, Western Blot and SDS-PAGE. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -70°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Note: Thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation by incubating the protein at 37ºC for 48h, with no obvious degradation or precipitation observed. Protein loss is <5% within the expiration date under appropriate storage conditions.
Physical State:
Supplied as lyophilized powder from PBS, pH 7.4, 5% Trehalose. Reconstitute with sterile ddH2O to a concentration of 0.1-1mg/ml. Do not vortex.
Purity:
≥95%.
Shipping Conditions:
Blue Ice
Storage Conditions:
-70[o]C
Supplier:
United States Biological
Type:
Proteins, Peptides, Small Molecules & Other Biomolecules:Recombinant Proteins