Bikunin (EDC1, HI30, Trypstatin, ITILC)
Product Sizes
100ug
£882.00
052819-100UG
About this Product
- SKU:
- 052819
- Application:
- Western Blot
- Clonality:
- Polyclonal
- Extra Details:
- Bikunin (also known as I alpha I light chain, EDC1, HI30 and Trypstatin) is a secreted, 35-45kD proteoglycan member of the kunin family of serine protease inhibitors. It is expressed by hepatocytes, amniotic epithelium and mesothelial cells. Bikunin possesses both sulfated and nonsulfated GAG sequences. The nonsulfated sequences are known to form ester linkages with two 80kD "heavy" chains in the hepatocyte Golgi, forming 180-200kD I alpha I. This molecule participates in the formation of hyaluronan-containing ECM. In addition, one of the H chains of I alpha I can be displaced by TSG6, creating an antiplasmin complex. Human bikunin is generated through cleavage of a precursor molecule termed AMBP. This AMBP should not be confused with AMBP-1, a 120-140kD adrenomedullin-binding protein that is also known as Complement Factor H. The AMBP precursor contains a 19 aa signal sequence, an N-terminal 183 aa A1M protein (aa 20-203), and the C-terminal serine protease inhibitor bikunin (aa 206-352). Bikunin possesses one GAG attachment site at Ser215, two Kunitz inhibitor domains (aa 231-281 and 287-337) and one utilized N-linked glycosylation site at Asn250. Although cleavage of AMBP in the Golgi may result in release of free bikunin, the 60-65kD AMBP precursor can also be released intact. Over aa 206-352, human bikunin shares 77% aa sequence identity with mouse bikunin. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 0.5ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Host:
- Sheep
- Immunogen:
- Recombinant protein corresponding to Ala206-Asn352 from human Bikunin, expressed in NS0 cells.
- Isotype:
- IgG
- Physical State:
- Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
- Purity:
- Purified by immunoaffinity chromatography.
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes human Bikunin.
- Source:
- human
- Storage Conditions:
- -20[o]C
- Supplier:
- United States Biological
- Type:
- Antibodies:Polyclonal Antibody
