NG2 (MCSP, Chondroitin Sulfate Proteoglycan NG2, Melanoma Associated Chondroitin Sulfate Proteoglycan)
Product Sizes
100ug
£926.00
052772-100UG
About this Product
- SKU:
- 052772
- Application:
- Western Blot
- Clonality:
- Polyclonal
- Extra Details:
- NG2 (neuron/glia-type 2 antigen; also MCSP/melanoma chondroitin sulfate proteoglycan) is a 250‑500 kDa type I integral membrane proteoglycan found on synantocytes (NG2+ glia), vascular pericytes, chondroblasts, macrophages and melanoma cells. It binds multiple components of the ECM, and serves as a ligand for alpha 3 beta 1 and alpha 4 beta 1 integrins. Mature human NG2 is 2293 amino acids (aa) in length. It contains a large 2195 aa extracellular region (aa 30‑2224) and a short 79 aa cytoplasmic tail. NG2 is variably glycanated. Without proteoglycan, NG2 is expressed as a 250 kDa glycoprotein. Juxtamembrane proteolysis generates a soluble ECD that noncovalently associates with the transmembrane fragment. Over aa 1583‑2224, human NG2 is 81% aa identical to both mouse and canine NG2. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: 0.5ug/ml using lysates of A375 human melanoma cell line and SK-Mel-28 human malignant melanoma cell line under under reducing condtions. Knockout Validated: NG2/MCSP is specifically detected in HeLa human cervical epithelial carcinoma parental cell line but is not detectable in NG2/MCSP knockout HeLa cell line. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Host:
- Goat
- Immunogen:
- Recombinant protein corresponding to Ser1583-Ser2224 from human NG2/MCSP, expressed in NS0 cells.
- Isotype:
- IgG
- Physical State:
- Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
- Purity:
- Purified by immunoaffinity chromatography.
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes human NG2/MCSP. ~30% cross-reactivity with recombinant mouse CSPG4.
- Source:
- human
- Storage Conditions:
- -20[o]C
- Supplier:
- United States Biological
- Type:
- Antibodies:Polyclonal Antibody
