Actin (ACTA1)
Product Sizes
100ul
£639.00
052755-100UL
About this Product
- SKU:
- 052755
- Application:
- Western Blot, Immunohistochemistry, Immunofluorescence
- translate.label.attr.clone:
- C4
- Clonality:
- Monoclonal
- Extra Details:
- Actin is the most abundant protein found in virtually all eukaryotic cells. It is also one of the most highly-conserved proteins, differing by no more than 20% in species as diverse as algae and humans, making it an excellent choice for use as a loading control. It is the monomeric subunit of microfilaments, one of the three major components of the cytoskeleton, and of thin filaments, which are part of the contractile apparatus in muscle cells. Thus, actin participates in many important cellular functions, including muscle contraction, cell motility, cell division and cytokinesis, vesicle and organelle movement, cell signaling, and the establishment and maintenance of cell junctions and cell shape. Applications: Suitable for use in Immunofluorescence/Immunocytochemistry, Immunohistochemistry and Western Blot. Other applications have not been tested. Recommended Dilutions: Western Blot: 1:5000 Immunohistochemistry: 1:500 Immunocytochemistry: 1:200-1:500 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Host:
- Mouse
- Immunogen:
- Preparation of chicken gizzard actin
- Isotype:
- IgG1
- Physical State:
- Supplied as a liquid, 0.01% sodium azide.
- Purity:
- Ascites
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes endogenous levels of chicken ~42kD actin protein. Reacts with all actin isoforms.
- Source:
- chicken
- Storage Conditions:
- -20[o]C
- Supplier:
- United States Biological
- Type:
- Antibodies:Monoclonal Antibody
