Ganglioside GM1 (Monosialoganglioside GM1)
Product Sizes
100ug
£832.00
052717-100UG
About this Product
- SKU:
- 052717
- Application:
- Immunohistochemistry
- translate.label.attr.clone:
- DG1
- Clonality:
- Monoclonal
- Extra Details:
- Ganglioside GM1 is a monosialylated ganglioside and the prototypic ganglioside for those containing one sialic acid residue. It is found in a large variety of cells, including immune cells and neurons, and is enriched in lipid rafts in the cell membrane. It associates with growth factor receptors, including TrkA, TrkB, and the GDNF receptor complex containing Ret and GFRα, and is required for TrkA expression on the cell surface. Ganglioside GM1 interacts with other proteins to increase calcium influx, affecting various calcium-dependent processes, including inducing neuronal outgrowth during differentiation. Ganglioside GM1 acts as a receptor for cholera toxin, which binds to its oligosaccharide group, facilitating toxin cell entry into epithelial cells of the jejunum. Similarly, it is bound by the heat-labile enterotoxin from E. coli in the pathogenesis of traveler's diarrhea. Ganglioside GM1 sensitizes inactivated T cells to TNF-α-induced apoptosis and induces apoptosis of activated T cells even in the absence of TNF-α. Ganglioside GM1 is found at higher levels on T cells isolated from patients with renal cell carcinoma (RCC) compared with T cells from patients without cancer. Levels of ganglioside GM1 are decreased in the substantia nigra pars compacta in postmortem brain tissues from patients with Parkinson's disease. Ganglioside GM1 gangliosidosis, characterized by a deficiency in ganglioside GM1-β-galactosidase, the enzyme that degrades ganglioside GM1, leads to accumulation of the gangliosides GM1 and GA1 in neurons and can be fatal in infants. Applications: Suitable for use in ELISA, Dot Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: ELISA: 1:1000-1:2000 Immunohistochemistry: 1:50 Dot Blot: 1:500-1:1000 Optional dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Host:
- Mouse
- Immunogen:
- C. jejuni OH4384
- Isotype:
- IgG2b
- Physical State:
- Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide, 50% glycerol.
- Purity:
- Purified by Protein G affinity chromatography.
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes Ganglioside GM1. Does not react with Ganglioside GM2, Ganglioside GM3, Ganglioside GD1a or Ganglioside GD1B.
- Storage Conditions:
- -20[o]C
- Supplier:
- United States Biological
- Type:
- Antibodies:Monoclonal Antibody
