Histone H1
Product Sizes
100ug
£800.00
052703-100UG
About this Product
- SKU:
- 052703
- Application:
- Immunoprecipitation, ChIP, Western Blot
- translate.label.attr.clone:
- 1G6
- Clonality:
- Monoclonal
- Extra Details:
- Histone H1 (UniProt: P02255; also known as H1) is encoded by the His 1 gene (Gene ID: 318854) in Drosophila species. Histone H1 is a linker histone that is involved in the organization and maintenance of chromatin higher order structure and plays an active role in the control of DNA replication and cell proliferation. The linker histone H1 family members are a key component of chromatin and bind to the nucleosomal core particle around the DNA entry and exit sites. Histone H1 is essential for the condensation of nucleosome chains into higher-order structures. In metazoans, H1 histones are smaller in size with about 200 amino acids. They have a short N-terminal tail, a central globular domain, and a long basic C-terminal tail. The central globular domain is shown to be highly conserved among all H1 subtypes. Manipulating levels of H1 histone can lead to both up- and down-regulation of specific genes. Depletion of H1 results in a massively altered chromosome structure with loss of chromosome banding. In Drosophila, a 20% reduction in H1 content leads to cessation of growth beyond the larval stage. Histone H1 undergoes phosphorylation mainly in the tail region, especially the C-terminal tail, where several {(S/T)-P-X-(K/R)} motifs are located that are recognized by cyclin-dependent kinases (CDKs). Phosphorylation levels are reported to be lowest during the G1 phase of the cell cycle and increase during S phase and reach maximum levels in mitosis. Applications: Suitable for use in Western Blot, Immunoprecipitation and ChIP. Other applications not tested. Recommended Dilution: Western Blot: 1:500 detected Histone H1 in Drosophila S2 cell lysate. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Host:
- Mouse
- Immunogen:
- GST-tagged recombinant protein fragment corresponding to 148aa from the C-terminal half of Drosophila Histone H1.
- Isotype:
- IgG1,k
- Physical State:
- Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
- Purity:
- Purified
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes Drosophila Histone H1.
- Source:
- drosophila
- Storage Conditions:
- -20[o]C
- Supplier:
- United States Biological
- Type:
- Antibodies:Monoclonal Antibody
