VEGFR1 (Vascular Endothelial Growth Factor Receptor 1, Flt-1)
Product Sizes
100ug
£902.00
052630-100UG
About this Product
- SKU:
- 052630
- Application:
- Flow Cytometry, Western Blot
- translate.label.attr.clone:
- 25H11
- Clonality:
- Monoclonal
- Extra Details:
- Vascular Endothelial Growth Factor Receptor 1 (VEGFR1) is a receptor tyrosine kinase that is expressed primarily on endothelial cells and plays a role in vasculogenesis and angiogenesis. A soluble variant of VEGFR1 was also reported to bind VEGF and PlGF with high affinity and function as a potent VEGF antagonist. Applications: Suitable for use in Western Blot and Flow Cytometry. Other applications not tested. Recommended Dilutions: Western Blot: 1ug/ml using recombinant human VEGFR1/Flt-1 Fc Chimera under non-reducing conditions only. Flow Cytometry: 2.5ug/10e6 cells in HUVEC human umbilical vein endothelial cells. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to Ser27-His687 from human VEGFR1, expressed in Sf21 cells.
- Isotype:
- IgG1
- Physical State:
- Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.
- Purity:
- Purified by Protein G affinity chromatography from hybridoma culture supernatant.
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes human VEGFR1. Does not cross-react with recombinant mouse VEGFR1 or recombinant human VEGFR2, VEGFR3 or VEGFR4.
- Source:
- human
- Storage Conditions:
- -20[o]C
- Supplier:
- United States Biological
- Type:
- Antibodies:Monoclonal Antibody
