DAG Kinase Alpha (DGKA, Diacylglycerol Kinase alpha, 80 kDa Diacylglycerol Kinase, Diglyceride Kinase alpha, DGK-alpha)
Product Sizes
100ug
£800.00
052629-100UG
About this Product
- SKU:
- 052629
- Application:
- Western Blot, Immunohistochemistry
- translate.label.attr.clone:
- 10D6.1
- Clonality:
- Monoclonal
- Extra Details:
- Diacylglycerol kinase alpha (UniProt: P23743; also known as EC:2.7.1.107, DAG kinase alpha, 80 kDa diacylglycerol kinase, Diglyceride kinase alpha, DGK-alpha) is encoded by the DGKA (also known as DAGK, DAGK1) gene (Gene ID: 1606) in human. Diacylglycerol kinase is a lipid kinase that converts diacylglycerol to phosphatidic acid, and regulates the activity of many enzymes including protein kinase C, phosphatidylinositol 4-phosphate 5-kinase, and mTOR. Thus far ten mammalian subtypes of DAG kinase have been reported. All DAG kinase with the exception of beta isoform are localized in cytoplasm in several cells, but some translocate to the plasma membrane upon activation. DAG kinase acts as a modulator, which competes with protein kinase C for diacylglycerol in intracellular signaling pathways. DAG kinase also plays a role in the resynthesis of phosphatidylinositols and phosphorylating diacylglycerol to phosphatidic acid. Most DAG kinases, with the exception of theta, contain two cysteine-rich regions (C1A and C1B domains) in the regulatory domain of the N-terminal half of the molecule. In DAK kinase alpha the C1 domain closest to the catalytic domain has an extended region of fifteen amino acids that is not present in C1 domains of other DAG kinases. DAG kinase alpha can be activated by the phosphorylation of Tyr335, which occurs during T cell activation or following exposure of cells to hepatocyte growth factor. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 1ug/ml detected DAG Kinase Alpha in 10ug of Jurkat cell lysate. Immunohistochemistry (paraffin): 1:50 detected DAG Kinase Alpha in human tonsil and human breast cancer tissue. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Host:
- Mouse
- Immunogen:
- GST/His-tagged recombinant protein fragment corresponding to the first 110 amino acids of human DAG kinase alpha.
- Isotype:
- IgG2a,k
- Physical State:
- Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
- Purity:
- Purified by Protein G affinity chromatography.
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes human DAG kinase alpha. Targets an epitope within the first 110 amino acids from the N-terminal region.
- Source:
- human
- Storage Conditions:
- -20[o]C
- Supplier:
- United States Biological
- Type:
- Antibodies:Monoclonal Antibody
