Chikungunya Virus E2
Product Sizes
100ul
£783.00
052623-100UL
About this Product
- SKU:
- 052623
- translate.label.attr.clone:
- CHK-152
- Clonality:
- Monoclonal
- Extra Details:
- Chikungunya virus (CHIKV) that causes distinctive polyarthritis or polyarthralgia with clinical features such as fever, maculopapular rash, and myalgia is transmitted by Aedes spp of mosquitoes. Immune compromised individuals may face serious complications, including encephalitis and mortality. CHIKV genome contains a single-stranded positive-sense RNA that encodes four non-structural proteins known as nsP1, nsP2, nsP3, and nsP4 and also five structural proteins that include a capsid protein, three envelope glycoproteins known as (E1, E2, and E3, and a small molecule known as 6K. The mature alphavirus particles express E1 and E2 heterodimers that form 80 trimeric spikes on the surface of the virion. The mature E2 protein contains three domains with immunoglobulin-like folds: N-terminal domain A, domain B at the tip; and the C-terminal domain C located proximal to the viral membrane. The ectodomain E1 protein consists of three domains known as D1, DII, and DIII. The DIII domain is an immunoglobulin-like domain connected to D1 and DII by a flexible linker. Both E1 and E2 proteins are responsible for virus entry into host cells. The E2 glycoprotein interacts with a cellular receptor, resulting in the virus internalization and the E1 glycoprotein mediates virus fusion to host cell under low pH conditions. Following the fusion of the viral envelope with the endosomal membrane, the viral genomic RNA is released into the cytoplasm and starts replicating. 052623 is a humanized antibody that blocks viral fusion and is shown to protect mice against CHIKV-induced mortality and disease. Applications: Suitable for use in Inhibition Assay, Neutralization and ELISA. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Host:
- Mouse
- Immunogen:
- Irf-7 deficient mice injected with La Reunion 2006 OPY-1 strain of Chikungunya virus (CHIKV-LR) and boosted with CHIKV virus-like particles and E2 protein.
- Isotype:
- IgG2a,k
- Physical State:
- Supplied as a liquid in PBS. No preservative added.
- Purity:
- Purified by Protein G affinity chromatography.
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes E2 protein in Chikungunya virus. Targets an epitope on A domain of E2.
- Storage Conditions:
- -20[o]C
- Supplier:
- United States Biological
- Type:
- Antibodies:Monoclonal Antibody
