Pro-Atrial Natriuretic Peptide
Product Sizes
100ug
£787.00
052609-100UG
About this Product
- SKU:
- 052609
- Application:
- Western Blot, Immunohistochemistry
- translate.label.attr.clone:
- 11E3.9
- Clonality:
- Monoclonal
- Extra Details:
- Natriuretic peptides A (UniProt P01160; also known as Atriopeptin, Cardiodilatin, Cardiodilatin-related peptide, Cardionatrin, CDD, CDD-ANF, CDP, Natriuretic peptide precursor A, Prepronatriodilatin) is encoded by the NPPA (also known as ANF, ANP, ATFB6, ATRST2, PND) gene (Gene ID 4878) in human. Atrial natriuretic peptide (ANP), also called atrial natriuretic factor, is a cardiac hormone that reduces high blood pressure by promoting salt excretion, decreasing blood volume, and relaxing vessel tension in a receptor-dependent manner. Deficiency in either ANP or its receptor causes spontaneous hypertension in knockout mice. ANP is initially synthesized in cardiac myocytes as a 153 a.a. precursor with a signal (a.a.1-25) and a propeptide (a.a. 26-123) sequence. The removal of the signal peptide yields pro-ANP (a.a. 26-151), which is further cleaved by the transmembrane cardiac serine protease Corin to yield mature ANP (a.a. 124-151), also known as atrial natriuretic factor (ANF). ANP is a short-lived molecule partly due to its proteolytic degradation by the kidney Neprilysin, also known as neutral endopeptidase 24.11. Applications: Suitable for use in Immunohistochemistry and Western Blot. Other applications not tested. Recommended Dilutions: Immunohistochemistry (FFPE): 1:1000 detected human Pro-Atrial Natriuretic Peptide in rat cardiac myocytes using heat-induced epitope retrieval (HIER). Western Blot: 1ug/mL detected human Pro-Atrial Natriuretic Peptide in 10ug of human heart tissue lysate. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Host:
- Mouse
- Immunogen:
- His-tagged recombinant protein corresponding to human Pro-Atrial Natriuretic Peptide. Epitope: Propeptide (aa26-123)
- Isotype:
- IgG1,k
- Physical State:
- Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
- Purity:
- Purified by Protein G affinity chromatography.
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes human Pro-Atrial Natriuretic Peptide. Expected to react with both prepro- and pro-atrial natriuretic peptide (ANP), but not mature ANP (aa124-151 of prepro sequence). Species Crossreactivity: rat
- Source:
- human
- Storage Conditions:
- -20[o]C
- Supplier:
- United States Biological
- Type:
- Antibodies:Monoclonal Antibody
