Dynamin-1/2
Product Sizes
100ug
£812.00
052606-100UG
About this Product
- SKU:
- 052606
- Application:
- Western Blot, Immunofluorescence
- translate.label.attr.clone:
- Hudy-1
- Clonality:
- Monoclonal
- Extra Details:
- Dynamin-1 & Dynamin-2 belong to the dynamin superfamily of large GTPases. Dynamins are multidomain proteins consisting of a GTP-binding domain, a middle domain, and a GTP-effector domain. Dynamins also contain a pleckstrin homology (PH) domain which may facilitate membrane binding. Dynamin-1 is highly abundant in the brain particularly at synaptic contacts, whereas Dynamin-2 is ubiquitously expressed in non-neuronal cells. Dynamins are involved in receptor-mediated endocytosis of clathrin-coated vesicles. Dynamin-1 interacts with an array of cellular targets via its SH3 binding domain and is implicated in late stages of vesicle formation by forming oligomers around the “neck” of budding vesicles and inducing membrane fission; it may also play a role in cytoskeleton rearrangement and cytoskinesis. Dynamin-2 is involved in the formation of clathrin-coated vesicles but may also be involved in the recycling of receptors between endosomes and the trans-Golgi network. Applications: Suitable for use in Western Blot and Immunofluorescence. Other applications have not been tested. Recommended Dilutions: Western Blot: 0.5ug/ml detected Dynamin-1/2 on 10ug of rat brain tissue lysate. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Host:
- Mouse
- Immunogen:
- Full length recombinant protein corresponding to human Dynamin-1, expressed in transfected Sf9 cells.
- Isotype:
- IgG1,k
- Physical State:
- Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
- Purity:
- Purified by Protein G affinity chromatography.
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes both human Dynamin-1 and Dynamin-2. Species Crossreactivity: rat
- Source:
- human
- Storage Conditions:
- -20[o]C
- Supplier:
- United States Biological
- Type:
- Antibodies:Monoclonal Antibody
