Glutamine Synthetase (GS)
Product Sizes
100ul
£895.00
052591-100UL
About this Product
- SKU:
- 052591
- Application:
- Western Blot, Immunohistochemistry
- Clonality:
- Polyclonal
- Extra Details:
- Glutamine synthetase (GS) catalyzes the amidation of glutamate to form glutamine. In the brain, GS is considered as a key enzyme, participating in recycling of the excitatory neurotransmitter glutamate released from neurons and termination of the neurotransmitter signal, as well as in ammonia detoxification. GS activity is a useful marker for astrocytes and an important differentiation feature in the retina. Upregulation of GS is a hallmark of reactive astrocytosis and GS activity level increases following spinal cord injury. The GS expression in astrocytes is diminished in brains affected by Alzheimer′s disease, particularly in the vicinity of senile plaques. GS is upregulated in a subset of human hepatocellular carcinomas, suggesting that its expression may be related to tumor recurrence, since tumors expressing GS are independent of the extracellular glutamine supply. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:10,000 using rat brain sections. Western Blot: 1:10,000 using rat brain cytosolic fraction. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Host:
- Rabbit
- Immunogen:
- Snthetic peptide corresponding to aa357-373 from the C-terminal region of mouse glutamine synthetase (GS).
- Isotype:
- IgG
- Physical State:
- Suppllied as a liquid in 0.01M PBS, pH 7.4, 15mM sodium azide.
- Purity:
- Purified
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes rat glutamine synthetase (GS) at 45kD. Staining of Glutamine Synthetase in immunoblotting is specifically inhibited with the GS immunizing peptide (aa357-373 with N-terminally added lysine).
- Source:
- rat
- Storage Conditions:
- -20[o]C
- Supplier:
- United States Biological
- Type:
- Antibodies:Polyclonal Antibody
