Lipocalin 2 (HNL, NGAL, Neutrophil Gelatinase Associated Lipocalin, Oncogene 24p3, Neutrophil gelatinase-associated lipocalin precursor P25, alpha-2 microglobulin-related subunit of MMP-9) (Biotin)
Product Sizes
50ug
£960.00
052559-50UG
About this Product
- SKU:
- 052559
- Application:
- Western Blot
- Clonality:
- Polyclonal
- Extra Details:
- Lipocalin 2 has been implicated in a variety of processes including cell differentiation, tumorigenesis, and apoptosis (1-3). It binds a bacterial catecholate sidropore bound to ferric ion such as enterobactin with a subnanomolar dissociation constant (KD = 0.41nM) (4). The bound ferric enterobactin complex breaks down slowly in a month into dihydroxybenzoyl serine and dihydroxybenzoic acid (DHBA). It also binds to a ferric DHBA complex with much less KD values (7.9nM) (4). Secretion of Lipocalin 2 in immune cells increases by stimulation of Toll-like receptor as a acute phase response to infection. As a result, it acts as a potent bacteriostatic reagents by sequestering iron (5). Moreover, Lipocalin-2 can alter the invasive and metastatic behavior of Ras-transformed breast cancer cells in vitro and in vivo by reversing epithelial to mesenchymal transition inducing activity of Ras, through restoration of E-cadherin expression, via effects on the Ras-MAPK signaling pathway (6). In the kidney, Lipocalin 2-mediated iron trafficking may be involved in protection from renal injury, and it has been implicated as a marker for early kidney failure (7, 8). Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilutions: ELISA (Detection): 0.1-0.4ug/ml Western Blot: 0.1ug/ml with recombinant rat Lipocalin‑2/NGAL Optimal dilutions to be determined by the researcher. Matched Pair: Capture: L2499-18 Detection: 052559 Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Host:
- Goat
- Immunogen:
- Recombinant protein corresponding to Gln21-Asn198 from rat Lipocalin 2 expressed in NS0 cells
- Isotype:
- IgG
- Physical State:
- Supplied as a lyophilized powder from PBS, pH 7.4, BSA. Labeled with Biotin. Reconstitute with 250ul sterile PBS.
- Purity:
- Purified by immunoaffinity chromatography.
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes rat Lipocalin 2. Shows <0.4% crossreactivity in sandwich ELISAs with recombinant mouse Lipocalin 2 and <0.2% crossreactivity with recombinant human Lipocalin 1 and Lipocalin 2. Species Crossreactivity (Western Blot): mouse
- Source:
- rat
- Storage Conditions:
- -20[o]C
- Supplier:
- United States Biological
- Type:
- Antibodies:Polyclonal Antibody
