Granulocyte Macrophage Colony Stimulating Factor Receptor alpha (GM-CSF R alpha, CD116)
Product Sizes
100ug
£1010.00
052557-100UG
About this Product
- SKU:
- 052557
- Application:
- Flow Cytometry
- translate.label.attr.clone:
- 25G14
- Clonality:
- Monoclonal
- Extra Details:
- Applications: Suitable for use in Flow Cytometry, Neutralization and Immunocytochemistry. Other applications not tested. Recommended Dilution: Flow Cytometry: 2.5ug/10e6 cells. Validated using J774A.1 mouse reticulum cell sarcoma macrophage cell line Immunocytochemistry: 8-25ug/ml in immersion fixed J774A.1 mouse reticulum cell sarcoma macrophage cell line Neutralization: Measured by its ability to neutralize GM‑CSF-induced proliferation in the DA3 mouse myeloma cell line. The Neutralization Dose (ND50) is typically 0.02-0.12ug/ml in the presence of 0.1ng/ml recombinant mouse GM‑CSF. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Host:
- Rat
- Immunogen:
- Recombinant protein corresponding to Leu30-Pro327 from mouse GM-CSF R alpha, expressed in NS0 cells.
- Isotype:
- IgG2a
- Physical State:
- Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
- Purity:
- Purified by Protein G affinity chromatography from culture supernatant. Endotoxin: ≤0.1EU/ug (LAL).
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes mouse GM-CSF R alpha. Does not react with recombinant human GM-CSF R alpha or beta.
- Source:
- mouse
- Storage Conditions:
- -20[o]C
- Supplier:
- United States Biological
- Type:
- Antibodies:Monoclonal Antibody
