Synaptopodin (SYNPO, KIAA1029)
Product Sizes
200ul
£473.00
052507-200UL
About this Product
- SKU:
- 052507
- Application:
- Western Blot, Immunohistochemistry, Immunofluorescence
- translate.label.attr.clone:
- 5F198
- Clonality:
- Monoclonal
- Extra Details:
- Synaptopodin, a prolin-rich actin-binding protein with 2 binding sites for actin, represents a new class of actin-binding proteins which has first been localized in podocytes and a subset of telencephalic postsynaptic densities. In human tissue synaptopodin has a molecular weight of 73.7kD and pI of 9.38 (calculated from sequence data); in mouse the corresponding data are 74kD, pI 9.27. In SDS-PAGE the antigen appears as 100kD polypeptide in brain and 110kD polypeptide in kidney (the difference might be attributed to posttranslational modifications). Applications: Suitable for use in Immunofluorescence/Immunocytochemistry, Western Blot and Immunohistochemistry. Other applications have not been tested. Recommended Dilution: Immunohistochemistry (Frozen): 1:50-1:200 (0.25-1ug/ml) Immunohistochemistry (paraffin): 1:50-1:200 (0.25-1ug/ml) Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Host:
- Mouse
- Immunogen:
- Isolated rat kidney glomeruli.
- Isotype:
- IgG1
- Physical State:
- Supplied as a liquid in PBS, pH 7.4, 0.5% BSA, 0.09% sodium azide. For antibody in lyophilized powder format see S9500-74.
- Purity:
- Purified by affinity chromatography.
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes rat Synaptopodin at ~44kD (degradation product). Species Crossreactivity: human, bovine, guinea pig, mouse and gerbil. Does not recognize rabbit, frog or chicken. Recognizes differentiated podocytes (glomerular visceral epithelial cells) in vivo and in vitro (weaker additional reaction with arterial endothelial cells); co-localizes with a-actinin. Does not react with parietal cells. Rea
- Source:
- rat
- Storage Conditions:
- -20[o]C
- Supplier:
- United States Biological
- Type:
- Antibodies:Monoclonal Antibody
