IL-23R (Interleukin 23 Receptor)
Product Sizes
100ug
£767.00
052267-100UG
About this Product
- SKU:
- 052267
- Application:
- Western Blot
- translate.label.attr.clone:
- 25A1
- Clonality:
- Monoclonal
- Extra Details:
- Interleukin 23 (IL-23) is a heterodimeric cytokine composed of two disulfide-linked subunits, a p19 subunit that is unique to IL-23, and a p40 subunit that is shared with IL-12 (1-5). The functional IL-23 receptor complex consists of two receptor subunits, the IL-12 receptor beta 1 subunit (IL-12R beta 1) and the IL-23-specific receptor subunit (IL-23R) (3). Human IL-23R cDNA encodes a 629 amino acids (aa) type I transmembrane protein with a 23 aa residue signal peptide, a 330 aa residue extracellular domain, a 23 aa residue transmembrane domain and a 253 aa residue cytoplasmic region. IL-23 R shares structural features with the IL-12R beta 2, including an N-terminal Ig-like domain, two cytokine receptor domains and multiple glycosylation sites in the extracellular domain. IL-23R lacks the three extracellular membrane-proximal fibronectin-type III domains present on IL-12R beta 2. IL-23R has a WQPWS sequence in the transmembrane-proximal cytokine receptor domain similar to the cytokine receptor signature WSXWS motif (6). The cytoplasmic region of IL-23R has three potential Src homology 2 domain-binding sites and two potential Stat-binding sites. The gene for human IL-23R is located on human chromosome 1 within 150 kb of IL-12R beta 2. Based on quantitative real-time PCR, human IL-23R mRNA is expressed in a human Th1 and Th0 clone as well as several NK cell lines and clones. Low but detectable levels of IL-23R mRNA is also expressed in EBV-transformed B cells and activated PBMC. IL-23 initiates a signal transduction cascade similar to that of IL-12, and involves Jak2, Tyk2, Stat1, Stat3, Stat4, and Stat5 (2). The Cynomolgus IL-23R shares 96%, 71% and 77% amino acid sequence identity to Human, mouse, and rat IL-23R, respectively. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: 2ug/ml using K562 human chronic myelogenous leukemia cell line, MCF‑7 human breast cancer cell line and Jurkat human acute T cell leukemia cell line. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Host:
- Mouse
- Immunogen:
- Human embryonic kidney cell HEK293-derived cynomolgus monkey IL-23R, Gly24-Asp353
- Isotype:
- IgG1
- Physical State:
- Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
- Purity:
- Purified by Protein G affinity chromatography from hybridoma culture supernatant.
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes cynomolgus monkey IL-23R in direct ELISAs and human IL-23R in Western blots.
- Storage Conditions:
- -20[o]C
- Supplier:
- United States Biological
- Type:
- Antibodies:Monoclonal Antibody
