WAVE2, phosphorylated (Tyr150) (Conserved Site)
Product Sizes
100ul
£576.00
052129-100UL
About this Product
- SKU:
- 052129
- Application:
- Western Blot
- Clonality:
- Polyclonal
- Extra Details:
- The Wiskott–Aldrich syndrome protein (WASP) family is involved in various pathways that regulate actin cytoskeletal organization. This family includes WASP, N-WASP, and three WAVE/SCAR isoforms, WAVE1, 2, and 3. WAVE proteins play key roles in actin-mediated cell events, such as membrane ruffling and lamellipodia formation. WAVEs contain an N-terminal WAVE homology domain, a basic domain, a Proline-rich region, and carboxy terminal verprolin, cofilin, and acidic (VCA) region. WAVEs are thought to act downstream of the Rac GTPase, connecting Rac activation to induction of Arp 2/3-mediated actin polymerization. Regulation of WAVE activity can occur through tyrosine phosphorylation. Src phosphorylation of WAVE1 at Tyr-125 enhances binding to the Arp2/3 complex, and is required for WAVE inhibition of Arp2/3-mediated stress fiber formation. By contrast, WAVE2 phosphorylation of Tyr-150 by Abl may enhance Arp2/3 complex actin nucleation and microspike formation in fibroblasts. Thus, site-specific tyrosine phosphorylation may be important for controlling specific activities of WAVE proteins. Applications: Suitable for use in Western Blot and Immunocytochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 1:1000, detects a ~78kD band corresponding to phosphorylated WAVE2 in K562 cells treated with pervanadate and a 80kD band corresponding to WAVE1 in HUVEC treated with pervanadate. It also recognizes an unidentified band near 50kD in K562 cells. Immunocytochemistry: 1:200 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Host:
- Rabbit
- Immunogen:
- Synthetic phosphopeptide corresponding to amino acid residues surrounding Tyr150 in human WAVE2 (KLH). This sequence has high homology with similar regions in rat and mouse WAVE2, and homology to the conserved sites in WAVE1 (Tyr151) and WAVE3 (Tyr151).
- Isotype:
- IgG
- Physical State:
- Supplied as a liquid in PBS, 1mg/ml BSA, 0.05% sodium azide, 50% glycerol
- Purity:
- Purified by immunoaffinity chromatography.
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes human WAVE2 phosphorylated at Tyr150. Species Crossreactivity: mouse and rat. 052129 was cross-adsorbed to phospho-WAVE (Tyr125) and unphosphorylated WAVE (Tyr150) peptides before affinity purification using phospho-WAVE (Tyr150) peptide
- Source:
- human
- Storage Conditions:
- -20[o]C
- Supplier:
- United States Biological
- Type:
- Antibodies:Polyclonal Antibody
