Tamm-Horsfall Glycoprotein (Uromucoid, THgp, Uromodulin, UMOD)
Product Sizes
100ug
£812.00
052074-100UG
About this Product
- SKU:
- 052074
- Application:
- Western Blot, Immunohistochemistry
- translate.label.attr.clone:
- 24I1
- Clonality:
- Monoclonal
- Extra Details:
- Tamm-Horsfall glycoprotein (THP, Uromodulin) is a 105-120kD urinary glycoprotein. It is secreted by renal tubule epithelium, acts as a binding protein for IL-1, TNF-alpha and C1q, activates resting monocytes, and promotes neutrophil phagocytosis. Uromodulin forms high molecular weight oligomers that line the kidney tubules. Mouse Uromodulin is GPI-linked. Its proprecursor is 619aa in length. It contains three EGF-like domains (aa28-148), a ZP domain that mediates oligomerization (aa335-590), and a cleavable C-terminal propeptide (aa619- 642). There are multiple splice variants. One shows an alternate start site at Met343, and there are two substitutions, a 17aa substitution for aa601-642, and a 166aa substitution for aa441- 642. Over aa25-614, mouse Uromodulin shares 78% and 89%aa identical to human and rat Uromodulin, respectively. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 0.5ug/ml using mouse kidney tissue Immunohistochemistry: 8-25ug/ml using immersion fixed frozen sections of mouse kidney Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Host:
- Rat
- Immunogen:
- Recombinant protein corresponding to Ser24-Ala618 from mouse Tamm-Horsfall Glycoprotein, expressed in NS0 cells.
- Isotype:
- IgG2a
- Physical State:
- Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.
- Purity:
- Purified by Protein G affinity chromatography from hybridoma culture supernatant.
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes mouse Tamm-Horsfall Glycoprotein. Shows ~100% crossreactivity with recombinant human Tamm-Horsfall Glycoprotein.
- Source:
- mouse
- Storage Conditions:
- -20[o]C
- Supplier:
- United States Biological
- Type:
- Antibodies:Monoclonal Antibody
