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Antibodies

CD146 (Mucin 18, MUC18 Glycoprotein, Melanoma Associated Antigen, Melanoma Cell Adhesion Molecule) (APC)

Product Sizes
100ug
£856.00
051992-100UG
About this Product
SKU:
051992
Application:
Flow Cytometry
translate.label.attr.clone:
7H217
Clonality:
Monoclonal
Extra Details:
Melanomia Cell Adhesion Molecule (MCAM), also known as CD146 and MUC18, is a putative Ig-superfamily adhesion molecule that is expressed on endothelial cells and a variety of tumor cells. MCAM is associated with tumor progression and metastasis and may be involved in embryonic neural development. An alternately spliced isoform of rat MCAM has a 40 residue deletion in the cytoplasmic domain. Within the extracellular region, rat MCAM shares 72% and 90% amino acid sequence identity with human and mouse MCAM, respectively. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 0.25-1ug/10e6 cells using PC-12 rat adrenal pheochromocytoma cell line. Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Host:
Mouse
Immunogen:
Recombinant protein corresponding to Arg19-Lys560 from rat CD146 expressed in NS0 cells. Species Sequence Homology: human; 72%, mouse; 90%
Isotype:
IgG2a
Physical State:
Supplied as a liquid in saline, 0.5% BSA, 0.09% sodium azide. Labeled with Allophycocyanin (APC).
Purity:
Purified by Protein A affinity chromatography from hybridoma culture supernatant.
Shipping Conditions:
Blue Ice
Specificity:
Recognizes rat CD146 (MCAM). Does not crossreact with recombinant human MCAM, BCAM or ALCAM.
Source:
rat
Storage Conditions:
4[o]C Do Not Freeze
Supplier:
United States Biological
Type:
Antibodies:Monoclonal Antibody