Mesothelin (APC)
Product Sizes
100Tests
£960.00
048684-100TESTS
About this Product
- SKU:
- 048684
- Application:
- Flow Cytometry
- translate.label.attr.clone:
- 8B35
- Clonality:
- Monoclonal
- Extra Details:
- Mesothelin is a 40kD glycosylphosphatidylinositol-anchored glycoprotein that is expressed on mesothelial cells in the pleura, pericardium and peritoneum and overexpressed in mesotheliomas and ovarian or pancreatic adenocarcinoma. Mesothelin is a product of the CAK-1 gene, which also encodes megakaryocyte-potentiating factor (MPF). Mature human mesothelin shares 60% amino acid identity with either mouse or rat mesothelin. Two variant forms exist; variant 1 has an eight amino acid (1kD) insertion and is rarely expressed, while variant 2 is a truncated form secreted in the majority of ovarian cancers but rarely found in normal individuals. Applications: Suitable for use in Flow Cytometry. Other applications have not been tested. Recommended Dilutions: Flow Cytometry: 10ul/10e6 cells. Validated using HeLa human cervical carcinoma cells. Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
- Host:
- Rat
- Immunogen:
- Recombinant protein corresponding to Glu296-Gly580 from human mesothelin, expressed in NS0 cells.
- Isotype:
- IgG2a
- Physical State:
- Supplied as a liquid in saline, 0.5% BSA, 0.09% sodium azide. Labeled with Allophycocyanin (APC).
- Purity:
- Purified by Protein G affinity chromatography from hybridoma culture supernatant.
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes human mesothelin.
- Source:
- human
- Storage Conditions:
- 4[o]C Do Not Freeze
- Supplier:
- United States Biological
- Type:
- Antibodies:Monoclonal Antibody
