Kininogen
Product Sizes
500ug
£1004.00
046188-500UG
About this Product
- SKU:
- 046188
- Application:
- Immunoprecipitation, Immunohistochemistry, Western Blot
- translate.label.attr.clone:
- 23K11
- Clonality:
- Monoclonal
- Extra Details:
- Kininogen, also known as alpha 2-thiol proteinase inhibitor, is a multi-function protein. There are two alternatively spliced forms, designated as the high molecular weight (HMW) and low MW (LMW) forms (1). The HMW form is synthesized as a 644 amino acid (aa) precursor with a signal peptide (aa 1‑18). The mature chain (aa 19‑644) is further processed into the heavy (aa 19‑380) and the light (aa 390‑644) chains. The active peptide bradykinin (aa 381‑389) is released, which has a variety of functions including muscle contraction, hypotension and inflammation. The heavy chain consists of three cystatin-like domains, which are responsible for inhibiting cysteine proteases. The light chain consists of a His-rich domain, which is associated with the clotting activity. In comparison to the HMW form, the LMW Kininogen (427 aa) has the same sequence in its heavy chain and bradykinin, but a different sequence in its light chain (aa 402‑427). The LMW form is not involved in blood clotting. Applications: Suitable for use in Immunohistochemistry, Immunoprecipitation and Western Blot. Other applications have not been tested Recommended Dilutions: Western Blot: 1ug/ml with recombinant human Kininogen high molecular weight (HKa) protein Immunohistochemistry: 0.5-25ug/ml in immersion fixed paraffin-embedded sections of human brain (substantia nigra) Immunoprecipitation: 25ug/ml with conditioned cell culture medium spiked with Recombinant Human Kininogen High Molecular Weight (HKa) Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to Lys438-Ser531 from human Kininogen, expressed in E. coli.
- Isotype:
- IgG2b
- Physical State:
- Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 1ml sterile PBS.
- Purity:
- Purified by Protein A affinity chromatography from hybridoma culture supernatant.
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes human Kininogen
- Source:
- human
- Storage Conditions:
- -20[o]C
- Supplier:
- United States Biological
- Type:
- Antibodies:Monoclonal Antibody
