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Antibodies

CD274 (B7-H1, PD-L1, Programmed Cell Death Ligand 1, B7 homolog 1) (Low Endotoxin)

Product Sizes
100ug
£448.00
045716-100UG
About this Product
SKU:
045716
Application:
Flow Cytometry
translate.label.attr.clone:
MIH6
Clonality:
Monoclonal
Extra Details:
CD274, also known as B7-H1 or programmed death ligand 1 (PD-L1), is a 40 kD type I transmembrane protein and a member of the B7 family within the immunoglobulin receptor superfamily. It is expressed on T cells, B cells, NK cells, dendritic cells, IFN-γ activated endothelial cells, and monocytes. B7-H1 is one of the ligands of PD-1. The interaction of B7-H1 with PD-1 plays an important role in the inhibition of T cell responses. Other studies have shown that B7-H1 is able to costimulate T cell growth and cytokine production. CD274 is involved in costimulation essential for T cell proliferation and production of IL-10 and IFN-γ, in an IL-2-dependent and a PD-1-independent manner. Its interaction with PD-1 inhibits T cell proliferation and cytokine production. Applications: Suitable for use in Flow Cytometry and Blocking. Other applications not tested. Recommended Dilutions: Flow Cytometry: ≤ 0.5ug/10e6 cells in 100ul volume or 100ul of whole blood Blocking: blocks the binding of mouse PD-L1 to PD-1 (CD279). Optimal dilutions to be determined by the researcher. Storage andStability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Host:
Rat
Immunogen:
Mouse PD-L1-transfected cells
Isotype:
IgG2a
Physical State:
Supplied as a liquid in PBS, pH 7.2. No preservative added.
Purity:
Purified by affinity chromatography. Endotoxin: <0.01EU/ug (<0.001ng/ug)
Shipping Conditions:
Blue Ice
Specificity:
Recognizes mouse CD274.
Source:
mouse
Storage Conditions:
-20[o]C
Type:
Antibodies:Monoclonal Antibody