Microtubule Associated Protein 2 (MAP2, MAP-2, MAP2A, MAP2B, MAP2C)
Product Sizes
100ul
£633.00
031388-100UL
About this Product
- SKU:
- 031388
- Application:
- Western Blot, Immunohistochemistry, Immunofluorescence
- Clonality:
- Polyclonal
- Extra Details:
- Microtubules are 25nm diameter protein rods found in most kinds of eukaryotic cells. They are polymerized from a dimeric subunit made of one a subunit and one b tubulin subunit. Microtubules are associated with a family of proteins called microtubule associated proteins (MAPs), which includes the protein τ (tau) and a group of proteins referred to as MAP1, MAP2, MAP3, MAP4 and MAP5 (Kindler & Gardner 1994). MAP2 is made up of two ~280 kDa apparent molecular weight bands referred to as MAP2a and MAP2b. A third lower molecular weight form, usually called MAP2c, corresponds to a pair of protein bands running at ~70 kDa on SDS-PAGE gels. All these MAP2 forms are derived from a single gene by alternate transcription, and all share a C-terminal sequence which includes either three or four microtubule binding peptide sequences, which are very similar to those found in the related microtubule binding protein τ (tau). MAP2 isoforms are expressed only in neuronal cells and specifically in the perikarya and dendrites of these cells. MAP2 has been recently shown to be the specific receptor for the neurosteroid pregnenolone (Fontaine-Lenore V. et al., 2006). Applications: Suitable for use in Immunofluorescence/Immunocytochemistry, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunofluorescence (IC): 1:10,000-1:20,000 Western Blot: 1:20,000 Immunohistochemistry: 1:5000-1:10,000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Host:
- Chicken/Avian
- Immunogen:
- Recombinant bovine MAP2 protein expressed in E. Coli.
- Isotype:
- IgY
- Physical State:
- Supplied as a liquid in PBS, 0.035% sodium azide.
- Purity:
- Purified
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes bovine MAP2. Species Crossreactivity: human, mouse and rat
- Source:
- bovine
- Storage Conditions:
- -20[o]C
- Supplier:
- United States Biological
- Type:
- Antibodies:Polyclonal Antibody
