Dermatopontin (DPT) BioAssay™ ELISA Kit (Mouse)
Product Sizes
96Tests
£927.00
024633-96TESTS
About this Product
- SKU:
- 024633
- Extra Details:
- Dermatopontin (DPT) BioAssay™ ELISA Kit (Mouse) is a sandwich enzyme immunoassay for in vitro quantitative measurement of DPT in mouse tissue homogenates, cell lysates and other biological fluids. Detection Range: 0.156-10ng/ml Sensitivity: 0.059ng/ml Intra-Assay CV: <10% Inter-Assay CV: <12% Kit Components: *024633A: Microtiter Strips, 1x96 wells, Pre-coated, ready to use *024633B: Standard, 2x1vial 024633C: Standard Diluent, 1x20ml *024633D: Detection Reagent A, 1x120ul *024633E: Detection Reagent B, 1x120ul 024633F: Assay Diluent A, 1x12ml 024633G: Assay Diluent B, 1x12ml 024633H: TMB Substrate, 1x9ml 024633K: Stop Solution, 1x6ml 024633L: Wash Buffer, 30x, 1x20ml Precaution: The Stop Solution (024633K) included for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material. Storage and Stability: Store *024633A, *024633B, *024633D and *024633E at -20°C. Store all the other components at 4°C. Unused kit is stable for 6 months. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap. Assay Procedure Summary: 1. Prepare all reagents, samples and standards. 2. Add 100ul standard or sample to each well. Incubate 2 hours at 37°C. 3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37°C. 4. Aspirate and wash 3 times. 5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37°C. 6. Aspirate and wash 5 times. 7. Add 90ul Substrate Solution. Incubate 15-25 minutes at 37°C. 8. Add 50ul Stop Solution. Read at 450nm immediately.
- Shipping Conditions:
- Blue Ice
- Storage Conditions:
- 4[o]C/-20[o]C
- Type:
- Assay Kits:Assays
