Cy5-Ubiquitin
Product Sizes
0.1 mg
£458.00
T3201-0.1MG
1 mg
£2010.00
T3201-1MG
About this Product
- SKU:
- T3201
- Buffer:
- See Datasheet
- Extra Details:
- Time resolved (TR)-FRET offers a sensitive, low background, stable and homogenous assay for determination of protein ubiquitination. Europium-ubiquitin and Cy5-ubiquitin are a pair of modified ubiquitin suitable for TR-FRET assays to assess formation of polyubiquitin chains, thereby reporting the activity of E2 and E3 enzymes in the reaction. The concentration and molar ratio of europium-ubiquitin and Cy5-ubiquitin in the RELAYTR Europium/Cy5 Ubiquitin Mix are optimized to achieve excellent signal-to-background ratio in ubiquitination reactions. Optimization of protein concentrations is often necessary to achieve a desirable signal-to-background ratio. Typical protein concentration ranges are: UbE1 (10-30 nM), E2 (25-250 nM) and E3 (10-250 nM).
- Molecular Weight:
- 9.5 kDa/9.1 kDa
- Shipping Conditions:
- Dry Ice
- Source:
- E. Coli Recombinant
- Storage Conditions:
- -80 0C
- Supplier:
- UBP Bio
- Type:
- Assay Kits: Assay Kits
