Fluorescein-Ubiquitin
Product Sizes
100 ug
£388.00
T2201-100UG
1 mg
£1660.00
T2201-1MG
About this Product
- SKU:
- T2201
- Additional Names:
- RELAYTR FRET Reagents & kits
- Buffer:
- See Datasheet
- Extra Details:
- Time resolved (TR)-FRET offers a sensitive, low background, stable and homogenous assay for determination of protein ubiquitination. Terbium-ubiquitin and fluorescein-ubiquitin are a pair of modified ubiquitin suitable for TR-FRET assays to assess formation of polyubiquitin chains, thereby reflecting the activity of E2 or E3 enzyme in the reaction. The concentration and molar ratio of terbium-ubiquitin and fluorescein-ubiquitin in the Terbium/Fluorescein Ubiquitin Mix are optimized to achieve excellent signal-to-background ratio in TR-FRET assays monitoring E3 autoubiquitination. Optimization of protein concentrations are often necessary to achieve a desirable signal-to-background ratio. Typical protein concentration ranges are: UbE1 (10-30 nM), E2 (25-250 nM) and E3 (10-250 nM).
- Molecular Weight:
- 9.5 kDa/9.0 kDa
- Shipping Conditions:
- Dry Ice
- Source:
- E. Coli Recombinant
- Storage Conditions:
- -80 0C
- Supplier:
- UBP Bio
- Type:
- Assay Kits: Assay Kits
