6xHis-TEV protease
Product Sizes
0.2 mg
£353.00
P1001-0.2MG
1 mg
£610.00
P1001-1MG
10 mg
£3387.00
P1001-10MG
About this Product
- SKU:
- P1001
- Buffer:
- 100 mM Tris, pH 8.5 at 4 0C, 500 mM NaCl, 5mM DTT, 0.5 mM EDTA and 50% Glycerol
- Extra Details:
- Tobacco Etch Virus (TEV) protease is a cysteine protease that is commonly used to remove fusion tags, including His, GST and MBP, at the N or C termini of recombinant proteins. TEV protease specifically recognizes a seven amino acid sequence Glu-Asn-Leu-Tyr-Phe-Gln-Gly/Ser (ENLYFQG/S) and cleaves between Gln and Gly/Ser under a wide range of conditions. Recombinant 6xHis-TEV was expressed in E. Coli and purified to near homogeneity. A Ser219Asn (S219N) substitution was introduced that has no effect on TEV protease activity, but inhibits auto-cleavage and thus, stabilizing TEV protease during incubation and long-term storage.
- Molecular Weight:
- 27 kDa
- Shipping Conditions:
- Dry Ice
- Source:
- E. coli recombinant
- Storage Conditions:
- - 80 0C
- Supplier:
- UBP Bio
- Type:
- Proteins, Peptides, Small Molecules & Other Biomolecules
