Linear polyUb Chain-binding Protein Identification / Validation Kit
Product Sizes
1 Kit
£657.00
J2520-1KIT
About this Product
- SKU:
- J2520
- Buffer:
- 20 mM Tris, 150 mM NaCl, 2 mM BME, 10% Glycerol
- Extra Details:
- Sumo1 Conjugation Kit is designed for assaying the conjugation of Sumo1 to protein substrates in vitro. In a protein sumoylation reaction, a Sumo protein is first activated by the Sumo activating enzyme SAE1/SAE2 (E1) in an ATP hydrolysis-dependent manner; activated Sumo is then transferred to the Sumo conjugating enzyme Ube2I (E2) by formation of a thiolester bond with the catalytic cysteine residue of Ube2I; Sumo-charged Ube2I can mediate protein sumoylation in the presence of an appropriate Sumo ligase (E3).
- Shipping Conditions:
- Dry Ice
- Source:
- E. coli recombinant
- Storage Conditions:
- - 80 0C
- Supplier:
- UBP Bio
- Type:
- Assay Kits: Assay Kits
