K48 polyUb Chain-binding Protein Identification / Validation Kit
Product Sizes
1 Kit
£657.00
J2500-1KIT
About this Product
- SKU:
- J2500
- Buffer:
- 20 mM Tris, 150 mM NaCl, 2 mM BME, 10% Glycerol
- Extra Details:
- This kit is designed for two purposes: 1) identification of proteins that specially bind K63 polyUb chains in whole cell or tissue lysates; 2) validation of direct interactions between K63 polyUb chains and their binding proteins. The non-cleavable 6xHis-tagged K63 polyUb chains (2-4) are resistant of deubiquitination when incubating with whole cell or tissue lysates, thus allowing maximally capture proteins that specifically bind K63 polyUb chains. After binding, the non-cleavable K63 polyUb chains and the binding proteins can be enriched by Nickel XPure Agarose Resin (included). The bound proteins and the non-cleavable K63 polyUb chains can be eluted from Ni resin using a buffer containing 250 mM imidazole (included) or by incubating with thrombin (not included). Either of the elution method can significantly reduce the amounts of non-specific binding proteins when compared with elution using SDS sample buffer.
- Shipping Conditions:
- Dry Ice
- Source:
- E. coli recombinant
- Storage Conditions:
- - 80 0C
- Supplier:
- UBP Bio
- Type:
- Assay Kits: Assay Kits
