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Boc-Leu-Arg-Arg-AMC (Boc-LRR-AMC)

Product Sizes
5 mg
£227.00
G3100-5MG
About this Product
SKU:
G3100
Buffer:
powder, no buffer
Extra Details:
Boc-LRR-AMC is a fluorogenic substrate for the trypsin – like activity of the 20S proteasome. Working concentrations of this substrate is 50-200 µM. The released AMC can be detected by a fluorimeter or plate reader at excitation/emission wavelengths of 380 nm/460 nm, respectively. When used to determine proteasome activity in cell lysates, cell lysates that are pre-treated with a proteasome inhibitor such as MG132, PS341 or epoxomicin can be used to determine the fluorescence contributed by other cellular proteases that cleave this substrate. Readings from proteasome inhibitor-treated lysates should be subtracted.
Molecular Weight:
773.8 g/mol
Shipping Conditions:
Ambient
Source:
chemical synthesis
Storage Conditions:
- 20 0C
Supplier:
UBP Bio
Type:
Proteins, Peptides, Small Molecules & Other Biomolecules: Substrates