Ac-Ala-Asn-Trp-AMC (Ac-ANW-AMC)
Product Sizes
25 mg
£600.00
G1121-25MG
About this Product
- SKU:
- G1121
- Buffer:
- powder, no buffer
- Extra Details:
- Z-LLE-AMC is a fluorogenic substrate for the peptidylglutamyl peptide hydrolyzing (PGPH) activity or the caspase-like activity of the 20S and 26S proteasomes. Working concentrations of this substrate is 50-200 µM. The released AMC can be detected by a fluorimeter or plate reader at excitation/emission wavelengths of 380 nm/460 nm, respectively. When used to determine proteasome activity in cell lysates, cell lysates that are pre-treated with a proteasome inhibitor such as MG132, PS341 or epoxomicin can be used to determine the fluorescence contributed by other cellular proteases that cleave this substrate. Readings from proteasome inhibitor-treated lysates should be subtracted.
- Molecular Weight:
- 664.8 g/mol
- Shipping Conditions:
- Ambient
- Source:
- chemical synthesis
- Storage Conditions:
- - 20 0C
- Supplier:
- UBP Bio
- Type:
- Proteins, Peptides, Small Molecules & Other Biomolecules: Substrates
