Fluorescein labeled K48-Ub2
Product Sizes
25 ug
£493.00
D1110-25UG
About this Product
- SKU:
- D1110
- Buffer:
- 20 mM Tris, 150 mM NaCl, 2 mM BME, 10% Glycerol
- Extra Details:
- This product contains an authentic K48-linked isopeptide bond, synthesized by using an enzymatic reaction containing UbE1, E2-25K, Ub(K48R) and Ub-fluorescein. Fluorescein is labeled on the C-terminus of Ub by mutating glycine 76 to a cysteine. In fluorescein labeled K48 Ub2, fluorescein is labeled on the proximal Ub. It is an excellent tool for monitoring deubiquitination that can be visualized by in-gel fluorescent detection of the production of fluorescein labeled monoUb (excitation at ~490 nm, and see product data sheet for examples). The assay is more quantitative and sensitive than immunoblotting of Ub. The detection limit is 10 ng or less.
- Molecular Weight:
- 17.6 kDa
- Shipping Conditions:
- Dry Ice
- Source:
- E. coli recombinant
- Storage Conditions:
- - 80 0C
- Supplier:
- UBP Bio
- Type:
- Proteins, Peptides, Small Molecules & Other Biomolecules
